ALK F1174L Reference Standard

Synonyms
CD246

Format
Genomic DNA

Description
The ALK F1174L Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays.

With this product you are able to:
• Analyze the sensitivity of your assay
• Gain certainty of the limit of detection
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

5% discount on online orders

Use 'ONLINE5'. *Terms apply

 
Catalog ID
Qty
Unit Price
HD660
$120.00
Bulk order discount

Availability: On Backorder

Dispatched within 24 hours

Total:
OR
Technical Information
Horizon DNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild-type standard to generate a lower allelic frequencies for limit of detection studies.

Horizon’s Base-Seq products are all derived from human cell lines, and are high molecular weight DNA. This format ensures that they may be applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

Technical Data

DNA Base Change
TTC→TTA
Cosmic ID
COSM28055
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.4

Product Information

Intended Use
For routine performance monitoring (Research Use Only)
Unit Size
1µg
Concentration
50ng/µl

General Information

Allelic Frequency
50%
Storage
4˚C
Expiry
24 months from the date of manufacture
Cell Line Background

HCT116

Quality Control

Allelic Frequency
Droplet Digital PCR™
Genotype
Sanger sequencing of locus specific PCR
Quality
Agarose gel electrophoresis, Quantitatitive PCR of GAPDH locus
Quantification
Spectrophotometry (A260)
Genotype
ALK (F1174L/+)

Characterization

Gel Image
ALK F1174L Reference Standard - Gel Image
DNA was run on a 1% agarose gel and shows a single high-molecular weight band
Chromatogram
ALK F1174L Reference Standard - Chromatogram
Chromatograph showing heterozygosity for the ALK F1174L mutation within ALK
Primers
PrimerSequenceSize
ForwardGATTTGCCCAGACTCAGCTC279
ReverseCCCCTGTCCAAGCCTAAAGT
SequencingCCCCTGTCCAAGCCTAAAGT--

Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 11394
PDF Download

Documents & Related Literature

Product Specification
PDF Download
Safety Data Sheet
PDF Download
Gene Editing Sequence File
PDF Download
Publications

Please browse our collection of publications below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Publication Information

TitleAuthorDateLink
KRAS mutation testing of tumours in adults with metastatic colorectal cancer: a systematic review and cost-effectiveness analysisWestwoodOct-14View
Eprobe-mediated screening system for somatic mutations in the KRAS locusATSUMIMar-15View
Higher Quality of Molecular Testing, an Unfulfilled Priority Results from External Quality Assessment for KRAS Mutation Testing in Colorectal CancerTembuyserMay-14View
Absence of BRAF exon 15 mutations in multiple myeloma and Waldenström’s macroglobulinemia questions its validity as a therapeutic target in plasma cell neoplasiasHatzimichaelMay-13View
Assessing standardization of molecular testing for non-small-cell lung cancer: results of a worldwide external quality assessment (EQA) scheme for EGFR mutation testingPattonJul-14View
KRAS, NRAS and BRAF mutations in Greek and Romanian patients with colorectal cancer: a cohort studyNegruDec-13View
Variation in pre-PCR processing of FFPE samples leads to discrepancies in BRAF and EGFR mutation detection: a diagnostic RING trialKappNov-14View
Tumor Homogeneity between Primary and Metastatic Sites for BRAF Status in Metastatic Melanoma Determined by Immunohistochemical and Molecular TestingBoursaultAug-13View
BRAF mutation testing in solid tumors: a methodological comparisonWeyantSep-14View
A comparison of methods for EGFR mutation testing in non-small cell lung cancerYoungDec-13View
Navigating the rapids: the development of regulated next-generation sequencing-based clinical trial assays and companion diagnosticsPantApr-14View
Highly sensitive detection of EGFR T790M on Ion Torrent PGM.DarwantoApr-13View
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada

$25.00

$45.00

FEDEX International Priority

EU Countries
(Excluding Italy)

€20.00

€36.00

FEDEX International Priority

UK

£18.00

£32.40

Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 

 

$25.00 - $80.00

 

$45.00 - $120.00

 

FEDEX International Priority

 

We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

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