AKT1 E17K Reference Standard

Synonyms
AKT, PKB, PRKBA, RAC

Format
FFPE

Description
The AKT1 E17K Reference Standard is a highly-characterized, biologically-relevant quality control material used to assess the performance of assays that detect somatic mutations, such as Sanger and qPCR sequencing assays. With this product you are able to: • Evaluate of workflow integrity from pre-analytical DNA extraction to post-analytical bioinformatics • Analyze the sensitivity of your workflow • Optimize and validate new cancer panels and routinely monitor the performance of your assay

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Catalog ID
Qty
Unit Price
HD167
$100.00
Bulk order discount
  • Buy 5 for $60.00 each and save 40%
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  • Buy 20 for $40.00 each and save 60%

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Technical Information
Horizon FFPE standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own cell line mixes. The standard is provided at an allelic frequency of 50%, and may be diluted using the corresponding wild type standard to generate a lower allelic frequencies for limit of detection studies and validation of your pre-analytical phase of the workflow. Horizon’s Base-Seq products are all derived from human cell lines. Using a proprietary method of mild fixation and homogenous paraffin embedding, we generate highly-reproducible and consistent FFPE curls. With any commercially available FFPE extraction protocol, our standards yield high molecular weight DNA. This format ensures that they may applied to a wide-range of assays including qPCR, Sanger sequencing, next-generation sequencing, mass array, and more.

Technical Data

DNA Base Change
GAG→AAG
NCBI Reference Assembly SNP
rs121434592
Cosmic ID
COSM33765
Fixation Method
10% Formalin
Section Size
15µm or 20µm
Cell Density
3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA Yield
≥ 400 ng using Promega Maxwell LEV Plus Extraction kit

Product Information

Intended Use
For routine performance monitoring (Research Use Only)
Unit Size
1 FFPE Section per vial
Extractable DNA
≥ 400 ng per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit

General Information

Allelic Frequency
50%
Storage
4˚C
Expiry
36 months from the date of manufacture
Cell Line Background

MCF10A

Quality Control

Allelic Frequency
Droplet Digital PCR™
Genotype
Sanger sequencing of locus specific PCR
Quality
Agarose gel electrophoresis, Quantitatitive PCR of GAPDH locus
Quantification
Quantifluor™
Genotype
AKT1 (E17K/+)

Characterization

Chromatogram
AKT1 E17K Reference Standard - Chromatogram
Chromatograph showing heterozygosity for the AKT1 E17K mutation within AKT1 exon 3 (SNP accession number: rs34409589)
Primers
PrimerSequenceSize
ForwardGCCTCCTGTCCATGGTACTC1198
ReverseGAGGGGAAGCAAAGGCTCTG
SequencingGCGCCACAGAGAAGTTGTTG--
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 8380
PDF Download
Batch ID: 19290
PDF Download

Documents & Related Literature

Product Specification
PDF Download
Safety Data Sheet
PDF Download
Gene Editing Sequence File
PDF Download
Publications

Please browse our collection of publications below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Publication Information

TitleAuthorDateLink
KRAS mutation testing of tumours in adults with metastatic colorectal cancer: a systematic review and cost-effectiveness analysisWestwoodOct-14View
Eprobe-mediated screening system for somatic mutations in the KRAS locusATSUMIMar-15View
Higher Quality of Molecular Testing, an Unfulfilled Priority Results from External Quality Assessment for KRAS Mutation Testing in Colorectal CancerTembuyserMay-14View
Absence of BRAF exon 15 mutations in multiple myeloma and Waldenström’s macroglobulinemia questions its validity as a therapeutic target in plasma cell neoplasiasHatzimichaelMay-13View
Assessing standardization of molecular testing for non-small-cell lung cancer: results of a worldwide external quality assessment (EQA) scheme for EGFR mutation testingPattonJul-14View
KRAS, NRAS and BRAF mutations in Greek and Romanian patients with colorectal cancer: a cohort studyNegruDec-13View
Variation in pre-PCR processing of FFPE samples leads to discrepancies in BRAF and EGFR mutation detection: a diagnostic RING trialKappNov-14View
Tumor Homogeneity between Primary and Metastatic Sites for BRAF Status in Metastatic Melanoma Determined by Immunohistochemical and Molecular TestingBoursaultAug-13View
BRAF mutation testing in solid tumors: a methodological comparisonWeyantSep-14View
A comparison of methods for EGFR mutation testing in non-small cell lung cancerYoungDec-13View
Navigating the rapids: the development of regulated next-generation sequencing-based clinical trial assays and companion diagnosticsPantApr-14View
Highly sensitive detection of EGFR T790M on Ion Torrent PGM.DarwantoApr-13View
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada

$25.00

$45.00

FEDEX International Priority

EU Countries
(Excluding Italy)

€20.00

€36.00

FEDEX International Priority

UK

£18.00

£32.40

Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 

 

$25.00 - $80.00

 

$45.00 - $120.00

 

FEDEX International Priority

 

We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

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