Quantitative Multiplex Formalin Compromised (Moderate) Reference Standard

Format
Formalin-Compromised DNA

Description
The Quantitative Multiplex Formalin Compromised DNA Reference Standards (Mild, Moderate and Severe) provide biologically-relevant quality control material which can be used to assess the performance of NGS assays. These Formalin-Compromised DNA Standards vary in levels of fragmentation and formalin damage providing the ability to challenge your assay.

With this product you are able to:
• Evaluate the effect of formalin on your assay
• Analyze the robustness of your workflow with multiple formats (DNA , FFPE and Formalin-Compromised DNA)
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

Catalog ID
Qty
Unit Price
HD799
$255.00
Bulk order discount
  • Buy 3 for $217.00 each and save 15%
  • Buy 5 for $204.00 each and save 20%

Availability: In stock

Dispatched within 24 hours

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Technical Information
Next generation sequencing (NGS) platforms give researchers much greater power when profiling tumor samples. However, the use of NGS presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s standards are appropriate for any NGS library preparation including whole-genome, whole-exome, custom capture and targeted amplicon panels. Horizon has developed this Quality-Seq (Q-Seq) NGS Reference Standard range to support the development and continued validation of Next Generation Sequencing platforms. The Quantitative Multiplex Reference Standard (QMRS) portfolio covers multiple endogenous SNPs, insertions and deletions. The QMRS includes 11 mutations at 0.8-24.5% allelic frequency in genomic DNA, FFPE and Formalin-Compromised DNA format. The multiple formats of the QMRS allow you to validate both the pre-analytical and analytical portion of your workflow using an isogenic reference standard. The FFPE format QMRS allows you to evaluate and optimize DNA extraction protocols without wasting precious patient samples. With the Formalin-Compromised format, the robustness of your NGS workflow may be tested to ensure that your library protocol and informatics pipeline can accommodate these highly degraded samples.

The QMRS standard is our most popular multiplex standard as it provides both a breadth of formats, but also a large range of allelic frequencies and onco-relevant mutants.

PLEASE NOTE: Batch 28255 and batch 27599 have been released under concession. The DIN (DNA integrity number) is slightly outside the defined range for moderate formalin-compromised DNA. For more information please contact technical@horizondiscovery.com

Technical Data

Genes Covered
BRAF, KIT, EGFR, KRAS, NRAS, PIK3CA
Cosmics
1,500+
Allelic Frequencies
1% - 24.5%
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.1

Product Information

Verified Mutations
ChromosomeGeneVariantExpected Allelic Frequency, %
7q34BRAFV600E10.50%
4q11-q12cKITD816V10.00%
7p12EGFRΔE746 - A7502.00%
7p12EGFRL858R3.00%
7p12EGFRT790M1.00%
7p12EGFRG719S24.50%
12p12.1KRASG13D15.00%
12p12.1KRASG12D6.00%
1p13.2NRASQ61K12.50%
3q26.3PIK3CAH1047R17.50%
3q26.3PIK3CAE545K9.00%
Presence confirmed in parental cell line
ChromosomeGeneVariantExpected Allelic Frequency, %
2p23ALKP1543S33%
1q25.2ABL2P986fs8%
5q21-q22APCR2714C33%
1p35.3ARID1AP1562fs33.50%
13q12.3BRCA2A1689fs33%
13q12.3CDX2V306fs41.50%
22q13.2EP300K291fs8%
4q31.3FBXW7G667fs33.50%
8p12FGFR1P150L8.50%
13q12FLT3V197A11.50%
2q33.3IDH1S261L10%
7q31METV237fs6.50%
3p21.3MLH1L323M8.50%
17q11.2NF1L626fs7.50%
22q12.2NF2P275fs8%
9q34.3NOTCH1P668S31.50%
1q21-q22NTRK15'UTR8.50%
4q12PDGFRAG426D33.50%
Unit Size
1µg
Concentration
50ng/µl

General Information

Storage
4˚C
Expiry
12 months from the date of manufacture
Cell Line Background

HCT116/RKO/SW48

Quality Control

Allelic Frequency
Droplet Digital PCR™
Genotype
Quantitatitive PCR of GAPDH locus
Quality
D1000 DNA ScreenTape assay
Quantification
Qubit dsDNA BR assay
Amplifiability
Quantitatitive PCR of GAPDH locus
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 21983
PDF Download
Batch ID: 26864
PDF Download
Batch ID: 28255
PDF Download
Batch ID: 27599
PDF Download

Documents & Related Literature

Product Specification
PDF Download
Safety Data Sheet
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada

$25.00

$45.00

FEDEX International Priority

EU Countries
(Excluding Italy)

€20.00

€36.00

FEDEX International Priority

UK

£18.00

£32.40

Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 

 

$25.00 - $80.00

 

$45.00 - $120.00

 

FEDEX International Priority

 

We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

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