Multiplex I cfDNA Reference Standard Set

Format
cfDNA

Description

The Multiplex I cfDNA Reference Standards are highly-characterized, biologically-relevant reference materials used to assess the performance of cfDNA assays that detect somatic mutations, including quantitative PCR, next-generation sequencing, and other novel assays.

With this product you are able to:
• Analyze the sensitivity and specificity of your assay
• Gain certainty of the limit of detection and limit of quantification
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

Catalog ID
Qty
Unit Price
HD780
$505.00
Bulk order discount
  • Buy 3 for $430.00 each and save 15%
  • Buy 5 for $404.00 each and save 21%

Availability: In stock

Dispatched within 24 hours

Total:
OR
Technical Information

High throughput platforms such as Next generation sequencing (NGS) and quantitative PCR (qPCR) platforms give researchers much greater power when profiling cfDNA samples. However, the use of these presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s cfDNA products are all derived from human cell lines, and are fragmented to an average size of 160 bp to closely resemble cfDNA extracted from human plasma.

This format ensures that they may applied to a wide-range of cfDNA NGS and qPCR assays including high-throughput platforms such as NGS and qPCR give researchers greater power when profiling cfDNA samples. Horizon has developed the cfDNA Reference Standard range to support the development and continued validation of cfDNA assays.

The Multiplex I cfDNA Reference Standard Set covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies. Batch-specific copy number values measured via ddPCR are provided with each Set.

cfDNA Application Note

Technical Data

Genes Covered
EGFR, KRAS, NRAS, PI3KCA
Cosmics
1,500+
Allelic Frequencies
5%, 1%, 0.1% and 0% (100% wild type)
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

Product Information

Fragment Size
160 bp
Unit Size
350 ng per vial
Concentration
20 ng/µl
Verified Mutations
100% Multiplex I Wild Type cfDNA Reference Standard (Part No.: HD776)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R0.00%
7p12EGFRΔE746 - A7500.00%
7p12EGFRT790M0.00%
7p12EGFRV769 - D770insASV0.00%
12p12.1KRASG12D0.00%
1p13.2NRASQ61K0.00%
1p13.2NRASA59T0.00%
3q26.3PIK3CAE545K0.00%
5% Multiplex I cfDNA Reference Standard  (Part No.: HD777)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R5.0%
7p12EGFRΔE746 - A7505.0%
7p12EGFRT790M5.0%
7p12EGFRV769 - D770insASV5.0%
12p12.1KRASG12D6.3%
1p13.2NRASQ61K6.3%
1p13.2NRASA59T6.3%
3q26.3PIK3CAE545K6.3%
1% Multiplex I cfDNA Reference Standard   (Part No.: HD778)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R1.0%
7p12EGFRΔE746 - A7501.0%
7p12EGFRT790M1.0%
7p12EGFRV769 - D770insASV1.0%
12p12.1KRASG12D1.3%
1p13.2NRASQ61K1.3%
1p13.2NRASA59T1.3%
3q26.3PIK3CAE545K1.3%
0.1%  Multiplex I cfDNA Reference Standard (Part No.: HD779)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R0.10%
7p12EGFRΔE746 - A7500.10%
7p12EGFRT790M0.10%
7p12EGFRV769 - D770insASV0.10%
12p12.1KRASG12D0.13%
1p13.2NRASQ61K0.13%
1p13.2NRASA59T0.13%
3q26.3PIK3CAE545K0.13%
Presence confirmed in parental cell line

For information on endogenous variants including EGFR G719S, download whole-exome sequencing data of the parental cell lines:

Exome Sequencing Data

General Information

Storage
4°C
Expiry
12 months from the date of manufacture
Cell Line Background

RKO/SW48

Quality Control

Fragmentation Size
D1000 DNA ScreenTape assay
Allelic Frequency
Droplet Digital™ PCR
Quantification
Qubit dsDNA BR Assay (post-fragmentation)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 16922
PDF Download
Batch ID: 18257
PDF Download
Batch ID: 21121
PDF Download
Batch ID: 22099
PDF Download
Batch ID: 25816
PDF Download
Batch ID: 26216
PDF Download
Batch ID: 26304
PDF Download
Batch ID: 27669
PDF Download
Batch ID: 28256
PDF Download

Documents & Related Literature

Product Insert
PDF Download
Product Specification
PDF Download
Safety Data Sheet
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada

$25.00

$45.00

FEDEX International Priority

EU Countries
(Excluding Italy)

€20.00

€36.00

FEDEX International Priority

UK

£18.00

£32.40

Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 

 

$25.00 - $80.00

 

$45.00 - $120.00

 

FEDEX International Priority

 

We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

Back to top