Multiplex I cfDNA Reference Standard Set

Format
cfDNA

Description

The Multiplex I cfDNA Reference Standards are highly-characterized, biologically-relevant reference materials used to assess the performance of cfDNA assays that detect somatic mutations, including quantitative PCR, next-generation sequencing, and other novel assays.

With this product you are able to:
• Analyze the sensitivity and specificity of your assay
• Gain certainty of the limit of detection and limit of quantification
• Optimize and validate new cancer panels and routinely monitor the performance of your assay

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Catalog ID: HD780
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Availability: In stock

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Technical Information

High throughput platforms such as Next generation sequencing (NGS) and quantitative PCR (qPCR) platforms give researchers much greater power when profiling cfDNA samples. However, the use of these presents challenges surrounding sample variability, platform bias, and potential failure to detect biomarkers. Horizon’s cfDNA products are all derived from human cell lines, and are fragmented to an average size of 160 bp to closely resemble cfDNA extracted from human plasma.

This format ensures that they may applied to a wide-range of cfDNA NGS and qPCR assays including high-throughput platforms such as NGS and qPCR give researchers greater power when profiling cfDNA samples. Horizon has developed the cfDNA Reference Standard range to support the development and continued validation of cfDNA assays.

The Multiplex I cfDNA Reference Standard Set covers multiple engineered single nucleotide variants (SNVs/SNPs) with 8 mutations at 5%, 1%, and 0.1% allelic frequencies. Batch-specific copy number values measured via ddPCR are provided with each Set.

cfDNA Application Note

Technical Data

Genes Covered
EGFR, KRAS, NRAS, PI3KCA
Cosmics
1,500+
Allelic Frequencies
5%, 1%, 0.1% and 0% (100% wild type)
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

Product Information

Fragment Size
160 bp
Unit Size
350 ng per vial
Concentration
20 ng/µl
Verified Mutations
100% Multiplex I Wild Type cfDNA Reference Standard (Part No.: HD776)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R0.00%
7p12EGFRΔE746 - A7500.00%
7p12EGFRT790M0.00%
7p12EGFRV769 - D770insASV0.00%
12p12.1KRASG12D0.00%
1p13.2NRASQ61K0.00%
1p13.2NRASA59T0.00%
3q26.3PIK3CAE545K0.00%
5% Multiplex I cfDNA Reference Standard  (Part No.: HD777)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R5.0%
7p12EGFRΔE746 - A7505.0%
7p12EGFRT790M5.0%
7p12EGFRV769 - D770insASV5.0%
12p12.1KRASG12D6.3%
1p13.2NRASQ61K6.3%
1p13.2NRASA59T6.3%
3q26.3PIK3CAE545K6.3%
1% Multiplex I cfDNA Reference Standard   (Part No.: HD778)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R1.0%
7p12EGFRΔE746 - A7501.0%
7p12EGFRT790M1.0%
7p12EGFRV769 - D770insASV1.0%
12p12.1KRASG12D1.3%
1p13.2NRASQ61K1.3%
1p13.2NRASA59T1.3%
3q26.3PIK3CAE545K1.3%
0.1%  Multiplex I cfDNA Reference Standard (Part No.: HD779)
ChromosomeGeneVariantExpected Allelic Frequency
7p12EGFRL858R0.10%
7p12EGFRΔE746 - A7500.10%
7p12EGFRT790M0.10%
7p12EGFRV769 - D770insASV0.10%
12p12.1KRASG12D0.13%
1p13.2NRASQ61K0.13%
1p13.2NRASA59T0.13%
3q26.3PIK3CAE545K0.13%
Presence confirmed in parental cell line

EGFR G719S (20% allelic frequency); download additional information on variants present in the parental cell lines:

Exome Sequencing Data

General Information

Storage
4°C
Expiry
12 months from the date of manufacture
Cell Line Background

RKO/SW48

Quality Control

Fragmentation Size
D1000 DNA ScreenTape assay
Allelic Frequency
Droplet Digital™ PCR
Quantification
Qubit dsDNA BR Assay (post-fragmentation)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 16922
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Batch ID: 18257
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Batch ID: 21121
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Batch ID: 22099
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Batch ID: 25816
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Batch ID: 26216
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Batch ID: 26304
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Batch ID: 27669
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Batch ID: 28256
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Batch ID: 28838
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Batch ID: 30458
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Documents & Related Literature

Product Insert
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Product Specification
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Safety Data Sheet
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Order & Delivery Information
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