Idylla™ Reference Pack

Format
FFPE

Description

This reference pack consists of 4 x HD200 (Quantitative Multiplex in an FFPE format) suitable for any KRAS/NRAS/BRAF Idylla™ system from Biocartis.

With this product you are able to:
• Save your precious samples by using this reference pack when a new batch of cartridges arrive
• Test the impact of changes in your software
• Gain confidence in your inter-laboratory reproducibility, using one control for all your assays

HD-Idylla200
$400.00

Availability: In stock

Dispatched within 24 hours

Bulk order discount
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Technical Information

Idylla™, Biocartis’ fully automated, real-time PCR based molecular diagnostics system, is designed to offer fast and easy access to clinical molecular diagnostic information, anywhere and anytime.

The Idylla™ Reference Pack consists of 4 x Quantitative Multiplex (HD200) Reference Standards in an FFPE format. The Quantitative Multiplex Reference Standard includes 11 mutations at 0.8-24.5% allelic frequency including: BRAF, NRAS, EGFR and KRAS variants as shown in the table below.

Horizon, in collaboration with Biocartis, has developed the Idylla™ Reference Pack, to support the ongoing quality control of the Idylla™ platforms. 

Available as FFPE, the Reference Pack allows you to monitor your assay without wasting precious patient samples. Regular use of the Idylla™ Reference Pack enables laboratories to monitor day-to-day workflow precision and to identify possible variability that may occur, such as lot-to-lot performance of cartridges, operator variation and software changes.


Technical Data

Genes Covered
BRAF, KIT, EGFR, KRAS, NRAS, PIK3CA
Cosmics
1,500+
Fixation Method
10% Formalin
Section Size
15µm or 20µm
Cell Density
3 x 108 cells per block. Approx. 3.5 x 105 cells per section
Expected DNA Yield
400-700ng DNA using Promega Maxwell LEV Plus Extraction kit

Product Information

Verified Mutations
ChromosomeGeneVariantAllelic Frequency
7q34BRAFV600E10.50%
4q11-q12cKITD816V10.00%
7p12EGFRΔE746 - A7502.00%
7p12EGFRL858R3.00%
7p12EGFRT790M1.00%
7p12EGFRG719S24.50%
12p12.1KRASG13D15.00%
12p12.1KRASG12D6.00%
1p13.2NRASQ61K12.50%
3q26.3PI3KCAH1047R17.50%
3q26.3PI3KCAE545K9.00%
Presence confirmed in parental cell line
ChromosomeGeneVariantAllelic Frequency
2p23ALKP1543S33%
1q25.2ABL2P986fs8%
5q21-q22APCR2714C33%
1p35.3ARID1AP1562fs33.50%
13q12.3BRCA2A1689fs33%
13q12.3CDX2V306fs41.50%
22q13.2EP300K291fs8%
4q31.3FBXW7G667fs33.50%
8p12FGFR1P150L8.50%
13q12FLT3S985fs10.50%
13q12FLT3V197A11.50%
2q33.3IDH1S261L10%
7q31METV237fs6.50%
3p21.3MLH1L323M8.50%
17q11.2NF1L626fs7.50%
22q12.2NF2P275fs8%
9q34.3NOTCH1P668S31.50%
1q21-q22NTRK15'UTR8.50%
4q12PDGFRAG426D33.50%
Unit Size
4 FFPE Sections
Extractable DNA
400-700ng DNA per section using Promega Maxwell LEV Plus FFPE DNA Extraction kit
Intended Use
For routine performance monitoring (Research Use Only)

General Information

Allelic Frequency
1% - 24.5%
Storage
4˚C
Expiry
48 months from the date of manufacture
Cell Line Background

HCT116/RKO/SW48

Quality Control

Genotype
Sanger sequencing of locus specific PCR
Quality
Agarose gel electrophoresis
Quantification
Quantifluor™
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