Human WHSC1 knockout cell line 1bp insertion

Gene Synonyms
MMSET, NSD2, REIIBP, TRX5, WHS

Description
HAP1 cell line, edited by CRISPR/Cas to contain a 1bp insertion in a coding exon of WHSC1.

This product includes the following:
• Genetically modified cell line
• Matched control
HZGHC001116c010
Academic
Commercial
$5,950.00

Availability: In stock

Within 7 business days

Bulk order discount
  • Highly characterized clonal human cell lines
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  • Wild type cell line included as a control
OR
Technical Information

Technical Data

Parental Cell Line
HAP1
Adherent Suspension
Adherent
Gene ID NCBI
7468
Genomic Location
chr4:1900763
Genome Assembly
hg38

General Information

Organism
Homo sapiens
Storage
LN vapor (less than -130°C)

Cell Line Characteristics

HGNC Symbol

WHSC1

Expression Level
101.53 TPM
The TPM expression value indicates this gene is
expressed
Guide RNA Sequence
TCGCGGTTCACCTCGCAGCT
Targeted Transcript
NM_001042424
Targeted Region
Exon 2
Mutation
1bp insertion
Transcript Plot Name

Quality Control

PCR FWD
TTGCTTACAAAGAAAATCAGACCCC
PCR BWD
GAAGTAAGATCTTTCAGCTTGTCGG
Sequencing Primer
TTGCTTACAAAGAAAATCAGACCCC
Sequencing Result
TACCTATCCTAGGTTTTAAATGTAATTGCTTTTTCTTTCTTTTTTCTTTTTTTTAATACCATAGTGTTCTAAGAACGGAAGCATCTGGGCTGGATGGAATTTAGCATCAAGCAGAGTCCCCTTTCTGTTCAGAGTGTTGTAAAGTGCATAAAGATGAAGCAGGCACCAGAAATCCTCGGCAGTGCCAACGGGAAGACTCCGAGCTTGCGAGGTGAACCGCGAGTGTTCTGTGTTCCTCAGCAAAGCCCAGCTCTCCAGTAGCCTGCAGGAGGGGGTCATGCAGAAGTTTAACGGCCACGACGCCCTGCCCTTTATTCCAGCCGACAAGCTGAAAGATCTTACTTC
Genotype
WHSC1 HAP1 knockout

Cell Culture

Biosafety Level
1
Media Type
IMDM, 10% FCS
Freeze Medium
IMDM, 20% FCS, 10% DMSO
Revival
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish.
Growth Properties
Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Protocols & Documents

Documents & Related Literature

Datasheet
PDF Download
Order & Delivery Information

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Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (USD)Price (EUR)
Price (GBP)Service

US & Canada

$200

 

 

FEDEX International Priority

EU Countries
(Excluding Italy)

$130

€99.00

£85.00

FEDEX International Priority

Rest of the World
(Excluding Japan, Israel, Hong Kong) 

$340

  

 

FEDEX International Priority

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