Horizon's X-MAN® (Mutant And Normal) cell lines are genetically engineered cell pairs containing defined genomic modifications, providing a model system for the study of the role of genes in cellular processes and drug responses. Cell lines are provided with a matched wild type control parental cell line ensuring phenotypes can be attributed directly to the genotype.
Using Horizon’s X-MAN Cell Lines you can:
Cell lines are provided as isogenic pairs – the modified mutant line and the originating wild type parental line.
All X-MAN Cell Lines are validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. To enable you to verify our results, we provide our sequencing result, including primer sequences.
We follow strict bio-banking procedures and our cells are regularly tested for contamination, including mycoplasma.
Our cell lines are shipped on dry ice but should be stored in standard mammalian cell culture storage conditions using liquid nitrogen for long-term storage.
Our Technical Support team is happy to provide a visual inspection of your culture if you send an image to firstname.lastname@example.org.
The primer sequences used for our internal genomic validation are provided on the Certificate of Analysis and Datasheet provided with the cell lines. We do not provide the PCR reaction conditions as these should be optimized within your own laboratory.
The ultimate proof that a cell line does not express the gene of interest is protein expression analysis by Western blot or similar. Unfortunately, due to the costs and time involved in optimising any new antibody,
Horizon cannot test protein expression in all its cell lines. As such we recommend that protein expression is assessed in cell lines prior to proceeding with downstream experiments.
If you would like to learn more about our products then please browse our dedicated Cell Line FAQ page here