HAP1 knockout cell lines

The single largest bank of isogenic cell lines with over 7,500 cell lines to choose from and trusted by academia, biotech, and pharma research labs. Cell models to suit any application, from TIDVAL to antibody validation, delivered in as few as 10 days.

HAP1 Parental Cell Lines available

RAB8A ( Human )

Entrez Gene 4218 entrezgene 4218
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RAB8A, member RAS oncogene family

Alias

MEL | RAB8

The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. This protein shares 97%, 96%, and 51% similarity with the dog RAB8, mouse MEL, and mouse YPT1 proteins, respectively and contains the 4 GTP/GDP-binding sites that are present in all the RAS proteins. The putative effector-binding site of this protein is similar to that of the RAB/YPT proteins. However, this protein contains a C-terminal CAAX motif that is characteristic of many RAS superfamily members but which is not found in YPT1 and the majority of RAB proteins. Although this gene was isolated as a transforming gene from a melanoma cell line, no linkage between MEL and malignant melanoma has been demonstrable. This oncogene is located 800 kb distal to MY09B on chromosome 19p13.1. [provided by RefSeq, Jul 2008].

Advantages of HAP1 knockout cell lines

  • Easily characterize knockout of your gene-of-interest in a biological context
  • Ready-made knockout cell lines are available for all non-essential human genes
  • Editing has been confirmed by Sanger sequencing of genomic DNA
  • Two independent knockout products for each gene, allowing second-clone analysis if more than one data point is required

Proven models

Our catalog of cell lines has become a gold-standard resourceciteab-award-cell-line-supplier-to-watch-in-cancer-research in antibody validation, cancer research, and early drug-discovery research. Horizon was recently recognized by CiteAb as the Cell Line Supplier to Watch in Cancer Research for 2024. Read more about the CiteAb award.

 

Why are HAP1 cells an ideal option for gene-edited cell line models?

Our HAP1 cell line database represents the synergy of two great systems - the amenability of human haploid HAP1s to gene editing and CRISPR-Cas9 technology. HAP1 is a human near-haploid cell line derived from the chronic myelogenous leukemia (CML) cell line KBM-7. KBM-7 cells were derived from a male patient and lack a Y chromosome. Therefore, haploid HAP1 cells contain a single X chromosome. For more details on how the HAP1 cell line was created and their full genotype, please see the references tab.

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HAP1 cell line properties include:
  • Sustained growth at single cell dilutions
  • Ease of transfection
  • Rapid doubling time
  • Not selected for ploidy status
    • Will have haploid and diploid populations

 

The majority of commonly used cell lines are diploid or even have more than two copies of any particular allele. When gene editing for loss of function mutations, each allele has to be modified for the phenotype to be expressed. The HAP1 cell line has the advantage of having one copy of each gene meaning you can be sure the edited allele will not be masked by addition alleles. With passaging, HAP1 cells will spontaneously diploidize over time. However, the knockout will not be affected by diploidization as it is a duplication of the edited allele that gives rise to the diploid state.

See our HAP1 publication list to see how the cell line has been applied for a wide range of biological processes and assays, such as DNA damage repair pathway, stress responses, disease modeling as well as antibody validation.

Limited use agreement

All cell lines engineered by Horizon Discovery are purchased under the terms of our Limited Use Label License.