Human PUSL1 knockout cell line 1bp insertion

Description
HAP1 cell line, edited by CRISPR/Cas to contain a 1bp insertion in a coding exon of PUSL1.

This product includes the following:
• Genetically modified cell line
• Matched control
HZGHC005912c010
Academic
Commercial
$5,950.00

Availability: In stock

Within 7 business days

Bulk order discount
  • Highly characterized clonal human cell lines
  • Strong IP position, supporting your freedom to operate
  • Wild type cell line included as a control
OR
Technical Information

Technical Data

Parental Cell Line
HAP1
Adherent Suspension
Adherent
Gene ID NCBI
126789
Genomic Location
chr1:1309490
Genome Assembly
hg38

General Information

Organism
Homo sapiens
Storage
LN vapor (less than -130°C)

Cell Line Characteristics

HGNC Symbol

PUSL1

Expression Level
18.33 TPM
The TPM expression value indicates this gene is
expressed
Guide RNA Sequence
ACGTGGCTGCGTGACGAGCG
Targeted Transcript
NM_153339
Targeted Region
Exon 4
Mutation
1bp insertion
Transcript Plot Name

Quality Control

PCR FWD
GAGATCTGGACAGACTTCCTTGTC
PCR BWD
CTAAAATCAGCACGTGACTTGAGGG
Sequencing Primer
GAGATCTGGACAGACTTCCTTGTC
Sequencing Result
CcATGTGaCaCCGCGGGCGGGCGGGGTCGTTCCTCCGGTGAGCTTtAcCTGCCGCCATAGGGTCCTGCGGGCCTTCCGAGTGCCCAGCGACTTCCACGCTTCGTCACGCAGCCACGTCCCGGACCTACCTGTACCGCCTGGCCACTGGCTGTCACCGGCGTGATGAGCTGCCGGTGTTTGAACGCAACCTATGCTGGACTCTCCCGGCAGAGTGAGTGTGGCCCTGACAGCGGGGAGGGGGCGGGCAGGCCGGCCCCACCCTCCTGACGGTCACCCTGGTCCCTGAAGCTGCCTGGATATGGTCGCCATGCAGGAAGCCGCCCAGCACCTCCTCGGCACACACGACT
Genotype
PUSL1 HAP1 knockout

Cell Culture

Biosafety Level
1
Media Type
IMDM, 10% FCS
Freeze Medium
IMDM, 20% FCS, 10% DMSO
Revival
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish.
Growth Properties
Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Protocols & Documents

Documents & Related Literature

Datasheet
PDF Download
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