Human PTTG1 (-/-) HCT116 Cell Line

Synonyms
EAP1, HPTTG, PTTG, PTTG1, Securin, TUTR1

Description
Homozygous knockout of PTTG1

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Catalog ID
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Unit Price
HD R02-043
$1,450.00

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Technical Information

Technical Data

Hugo Name

PTTG1

HD Derivation Slide
YES
Chromosome Number
near diploid with the modal number at 45 (62%) and polyploids occurring at 6.8%
Cell Line

HCT116

Functional Area
Cell Cycle
Primary Reference
Jallepalli et al. Securin is required for chromosomal stability in human cells. Cell 2001 v105 p445
Ready to Ship
<7 days

General Information

Parental ATCC ID
CCL-247
Tissue
Colon
Disease
Colorectal carcinoma
Gender
Male
Key Endogenous Mutations
CDKN2A, CTNNB1, KRAS, PIK3CA, MLH1
STR Profile
Amelogenin: X,Y ; CSF1PO: 7,10 ; D13S317: 10,12 ; D16S539: 11,13 ; D5S818: 10,11 ; D7S820: 11,12 ; THO1: 8,9 ; TPOX: 8,9 ; vWA: 17,22; ATCC
Organism
Homo sapiens

Cell Line Characteristics

Gene ID
9232
Link To NCBI
http://www.ncbi.nlm.nih.gov/gene?term=9232
Genomic Locus
5q35.1
CVT Selection Condition
G418 resistant – 0.4mg/ml

Quality Control

Genotype
PTTG1 (-/-)
Cell Number
1x10⁶

Cell Culture

Revival
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 75cm2 flask containing pre-warmed media.
Media Type
RPMI 1640 including 2mM L-glutamine and 25mM sodium bicarbonate, supplemented with 10% FBS.
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:10-1:20.
Biosafety Level
1
Freeze Medium
Complete growth media 45%; USDA approved FBS 50%; DMSO 5%
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FAQ

What are X-MAN Cell Lines?

Horizon's X-MAN® (Mutant And Normal) cell lines are genetically engineered cell pairs containing defined genomic modifications, providing a model system for the study of the role of genes in cellular processes and drug responses. Cell lines are provided with a matched wild type control parental cell line ensuring phenotypes can be attributed directly to the genotype.

What should I use X-MAN Cell Lines for?

Using Horizon’s X-MAN Cell Lines you can:

  • Study loss or gain of function mutations at the endogenous level
  • Initiate target identification and validation studies
  • Identify responsive or resistant patient populations in a human cell line model
  • Analyse expression, protein interactions and localization using endogenously tagged genes

Are mutant cell lines provided with a wild type control?

Cell lines are provided as isogenic pairs – the modified mutant line and the originating wild type parental line. 

How are X-MAN® Cell Lines validated?

All X-MAN Cell Lines are validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. To enable you to verify our results, we provide our sequencing result, including primer sequences.

How does Horizon confirm X-MAN® cell lines are free from contamination?

We follow strict bio-banking procedures and our cells are regularly tested for contamination, including mycoplasma.

Under what conditions should I store my X-MAN Cell Lines?

Our cell lines are shipped on dry ice but should be stored in standard mammalian cell culture storage conditions using liquid nitrogen for long-term storage.

I have never worked with these cells before.  How do I know if they look correct?

Our Technical Support team is happy to provide a visual inspection of your culture if you send an image to technical@horizondiscovery.com.

Do you provide the PCR conditions for your cell line genomic validation?

The primer sequences used for our internal genomic validation are provided on the Certificate of Analysis and Datasheet provided with the cell lines. We do not provide the PCR reaction conditions as these should be optimized within your own laboratory.

What other validations do you recommend to be performed?

The ultimate proof that a cell line does not express the gene of interest is protein expression analysis by Western blot or similar. Unfortunately, due to the costs and time involved in optimising any new antibody,

Horizon cannot test protein expression in all its cell lines. As such we recommend that protein expression is assessed in cell lines prior to proceeding with downstream experiments.

If you would like to learn more about our products then please browse our dedicated Cell Line FAQ page here

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TerritoryPrice (USD)Price (EUR)
Price (GBP)Service

US & Canada

$200

 

 

FEDEX International Priority

EU Countries
(Excluding Italy)

$130

€99.00

£85.00

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Rest of the World
(Excluding Japan, Israel, Hong Kong) 

$340

  

 

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