Human PI4K2A knockout cell line 7bp deletion

Gene Synonyms
PI4KII, PIK42A, RP11-548K23.6

Description
HAP1 cell line, edited by CRISPR/Cas to contain a 7bp deletion in a coding exon of PI4K2A.

This product includes the following:
• Genetically modified cell line
• Matched control
HZGHC002916c001
Academic
Commercial
$5,950.00

Availability: In stock

Within 7 business days

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  • Highly characterized clonal human cell lines
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  • Wild type cell line included as a control
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Technical Information

Technical Data

Parental Cell Line
HAP1
Adherent Suspension
Adherent
Gene ID NCBI
55361
Genomic Location
chr10:97651049
Genome Assembly
hg38

General Information

Organism
Homo sapiens
Storage
LN vapor (less than -130°C)

Cell Line Characteristics

HGNC Symbol

PI4K2A

Expression Level
12.41 TPM
The TPM expression value indicates this gene is
expressed
Guide RNA Sequence
GACTGCCTTGTCCTTAACCA
Targeted Transcript
NM_018425
Targeted Region
Exon 2
Mutation
7bp deletion
Transcript Plot Name

Quality Control

PCR FWD
GGCTATTTTGGTCAACTCGGATTTA
PCR BWD
ACTTAGGGTATAAATGACCCCACTG
Sequencing Primer
GGCTATTTTGGTCAACTCGGATTTA
Sequencing Result
CATTGCTGTCTTCAAACCCAAGAATGAAGAGCCCTATGGGCATCTTAATCCTAAGTGGACCAAGTGGCTGCAGAAGCTGTGCTGTCCTTGCTGCTTTGGCCGTGACTGCCTTGTCCTTAATATCTCTCAGAAGCAGGGGCCAGCCTGGTGGACCAAAAACTGGAACTCAACATTGTTCCCCGTACAAAGGTCAGTGACCCATCTCCAGGAAGCCTTACTGCCTGGCCACAGTTTTCCTGGGGCCTAAGCCCTGCTACATGTTAGTGGGACCTTGGGTCCAGTGGGGTCATTT
Genotype
PI4K2A HAP1 knockout

Cell Culture

Biosafety Level
1
Media Type
IMDM, 10% FCS
Freeze Medium
IMDM, 20% FCS, 10% DMSO
Revival
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish.
Growth Properties
Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Protocols & Documents

Documents & Related Literature

Datasheet
PDF Download
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