Human MAP2K1 (Q56P/+) RKO Cell Line

Synonyms
MAP2K1, MAPKK1, MEK1, MKK1, PRKMK1

HD 106-019
$1,950.00

Availability: In stock

Dispatched within 24 hours

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Technical Information

Technical Data

MYCO Test Type
Culture
Cell Line

RKO

Ready to Ship
<7 days

General Information

Tissue
Colon
Disease
Carcinoma
Gender
Female
Key Endogenous Mutations
BRAF, NF1, PIK3CA+
STR Profile
Amelogenin: X ; CSF1PO: 8, 10 ; D13S317: 8, 11 ; D16S539: 12, 13 ; D5S818: 11, 13 ; D7S820: 8, 10 ; THO1: 6, 10 ; TPOX:11 ; vWA: 15, 16, 17; ATCC
Organism
Homo sapiens

Cell Line Characteristics

Gene ID
2604
CVT Selection Condition
TBC

Quality Control

Genotype
MAP2K1 (Q56P/+)
Cell Number
1x10⁶

Cell Culture

Revival
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 75cm2 flask containing pre-warmed media.
Media Type
RPMI 1640 including 2mM L-glutamine and 25mM sodium bicarbonate, supplemented with 10% FBS.
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:6-1:15.
Biosafety Level
1
Freeze Medium
Complete growth media 45%; USDA approved FBS 50%; DMSO 5%
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