Human LRRK2 (G2019S/G2019S) iPSC-derived Neural Stem Cells

Synonyms
PARK8; RIPK7; ROCO2; AURA17; DARDARIN

ax0310
$1,280.00

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Technical Information
Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the R406W mutation (CGG>TGG) into the MAPT gene. This line is homozygous for the R406W mutation so both alleles contain the mutation. Click on the product images to see the data and further details. The R406W mutation in MAPT has been implicated in familial frontotemporal dementia and parkinsonism (Hutton et al., 1998; Behnam et al., 2015) and has been seen in patients with clinical presentation resembling Alzheimer's disease (Rademakers et al., 2003; Lindquist et al., 2008). The R406W mutation reduces the ability of MAPT/tau to bind to microtubules in vitro (Hong et al., 1998).

Technical Data

Genotype
LRRK2 (G2019S/G2019S)
Functional Area
Neurodegenerative Disease
Genetic Modification
GGG > GGA
Gene Copy Number
LRRK2: 1.87
SNP Frequency %
G2019S: 99.93%
Karyotype
Normal

General Information

Growth Properties
Adherent
Cell Number
1.5 x 106
Freeze Medium
Neural Expansion Medium (ax0030-500) with 10% DMSO
Method of Reprogramming
Episomal
Gene Editing Modification
Contains a puromycin resistance cassette (intronic)

Donor Information

Gender
Female
Age
64 years old
Ethnicity
Caucasian

Cell Culture

Biosafety Level
Biosafety Level 1
Cell Culture Image
Human LRRK2 (G2019S/G2019S) iPSC-derived Neural Stem Cells - Cell Culture Image

Expression of neural stem cell marker Nestin (red) and LRRK2 protein (green) in Human LRRK2 (G2019S/G2019S) iPSC-derived Neural Stem Cells

Human LRRK2 (G2019S/G2019S) iPSC-derived Neural Stem Cells - Cell Culture Image

DNA sequencing of Human LRRK2 (G2019S/G2019S) iPSCs shows that both alleles contain the G2019S mutation (GGC > AGC) at the locus. A silent mutation (underlined red) is introduced to prevent re-cutting of the gene edited region. This does not affect the amino acid sequence of the protein.

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US & Canada

$200

 

 

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EU Countries
(Excluding Italy)

$130

€99.00

£85.00

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Rest of the World
(Excluding Japan, Israel, Hong Kong) 

$340

  

 

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