Human CHEK2 (-/-); TP53 (+/+) HCT116 Cell Line

Synonyms
CDS1, CHEK2, CHK2, CHK2 ; TP53, hCds1, HuCds1, LFS1, LFS2, P53, p53, PP1425, RAD53, TRP53

Description
Homozygous knockout of CHEK2; TP53 wild type background
HD R02-017
Academic
Commercial
$5,950.00

Availability: In stock

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  • Highly characterized clonal human cell lines
  • Strong IP position, supporting your freedom to operate
  • Wild type cell line included as a control
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Technical Information

Technical Data

HD Derivation Slide
YES
Chromosome Number
near diploid with the modal number at 45 (62%) and polyploids occurring at 6.8%
MYCO Test Type
Culture
Cell Line

HCT116

Functional Area
Cell Cycle, DNA damage response, DNA repair, P53 signalling
Primary Reference
Jallepalli PV, (2003). The Chk2 tumor suppressor is not required for p53 responses in human cancer cells. J Biol Chem. 278(23):20475-20479.
Ready to Ship
<7 days

General Information

Tissue
Colon
Disease
Colorectal carcinoma
Gender
Male
Key Endogenous Mutations
CDKN2A, CTNNB1, KRAS, PIK3CA, MLH1
STR Profile
Amelogenin: X,Y ; CSF1PO: 7,10 ; D13S317: 10,12 ; D16S539: 11,13 ; D5S818: 10,11 ; D7S820: 11,12 ; THO1: 8,9 ; TPOX: 8,9 ; vWA: 17,22; ATCC
Organism
Homo sapiens

Cell Line Characteristics

Gene ID
11200;7157
Link To NCBI
http://www.ncbi.nlm.nih.gov/gene?term=11200 ; http://www.ncbi.nlm.nih.gov/gene?term=7157
Genomic Locus
22q12.1;17p13.1
CVT Selection Condition
G418 resistant – 0.4mg/ml

Quality Control

Genotype
CHEK2 (-/-); TP53 (+/+)
Cell Number
1x10⁶

Cell Culture

Revival
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 75cm2 flask containing pre-warmed media.
Media Type
RPMI 1640 including 2mM L-glutamine and 25mM sodium bicarbonate, supplemented with 10% FBS.
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:10-1:20.
Biosafety Level
1
Freeze Medium
Complete growth media 45%; USDA approved FBS 50%; DMSO 5%
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