Human BRCA2 (+/-) DLD-1 Cell Line

Synonyms
BRCA2, BRCC2, BROVCA2, FACD, FAD, FAD1, FANCB, FANCD, FANCD1, GLM3, PNCA2

Description
Heterozygous knockout of BRCA2

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Catalog ID
Qty
Unit Price
HD 105-027
$1,450.00

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Technical Information

Technical Data

Hugo Name

BRCA2

HD Derivation Slide
YES
Chromosome Number
This is a pseudodiploid human cell line with the modal chromosome number of 46, occurring in 86% of cells. The rate of polyploidy was high at 17.1%. The karyotype of the line was 46,XY,-2,+dir dup(2)(p13-p23). The Y chromosome was slightly longer than N22 and had a large segment of heterochromatic, fluorescent distal q arms
MYCO Test Type
Culture
Cell Line

DLD-1

Functional Area
DNA Repair
Primary Reference
Hucl, T., et al. (2008) A syngeneic variance library for functional annotation of human variation: application to BRCA2. Cancer Res. 68(13):5023-5030.
Ready to Ship
<7 days

General Information

Parental ATCC ID
CCL-221
Tissue
Colon
Disease
Colorectal adenocarcinoma Dukes Type C
Gender
Male
Key Endogenous Mutations
APC, BRCA2, FAM123B, KRAS, MSH6, PI3KA, TP53,
STR Profile
Amelogenin: X,Y ; CSF1PO: 11,12 ; D13S317: 8,11 ; D16S539: 12,13 ; D5S818: 13 ; D7S820: 10,12 ; THO1: 7,9.3 ; TPOX: 8,11 ; vWA: 18,19; ATCC
Organism
Homo sapiens

Cell Line Characteristics

Gene ID
675
Link To NCBI
http://www.ncbi.nlm.nih.gov/gene?term=675
Genomic Locus
13q12.3
CVT Selection Condition
No antibiotic resistance

Quality Control

Genotype
BRCA2 (+/-)
Cell Number
1.5x10⁶

Cell Culture

Revival
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 75cm2 flask containing pre-warmed media.
Media Type
RPMI 1640 including 2mM L-glutamine and 25mM sodium bicarbonate, supplemented with 10% FBS.
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:6-1:10.
Biosafety Level
1
Freeze Medium
Complete growth media 45%; USDA approved FBS 50%; DMSO 5%
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$200

 

 

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$130

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$340

  

 

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