Proving that the field of RNA-guided nucleases for gene editing is only in its infancy, a novel non-Cas9 CRISPR system isolated from Francisella novicida strain U112 by Zetsche et al in September 2015.
The system works in a similar fashion to CRISPR-Cas9, with a nuclease being recruited to a target site by sequence homology, However it differs from Cas9 in that:
The discovery of this system further expands the genome editors tool box - potentially making available target sites that, due to PAM constraints, would not be available to Cas9. Further to this the authors hypothesise that the nature of Cpf1 cuts, being both sticky and distal to the PAM site may have some added advantages for homology directed repair.
Watch one of our genome editing webinars:
|An Introduction to CRISPR Gene Editing||Getting started with CRISPR genome editing|
|Planning a genome editing experiment||Maximise your chances of Gene Editing Success|
|Modifying human cell lines with CRISPR||Human cell lines, genomic modification and editing efficiencies|