This model contains a deletion of the Fragile X mental retardation 1 gene (Fmr1). Mutations in Fmr1 result in Fragile X syndrome, the leading monogenic cause of autism, making this rat useful for the study of both Fragile X syndrome and autism.
• This model was created in collaboration with Autism Speaks and is currently undergoing phenotypic characterization by Dr. Richard Paylor at Baylor College of Medicine • Preliminary results suggest Fmr1 knockout rats possess perseverative chewing behavior and decreased juvenile play • Homozygous knockout rats exhibit complete loss of target protein as demonstrated by Western blot • An expansion of CGG trinucleotide repeat in Fmr1 has been implicated in Fragile X syndrome • Background Strain: Sprague-Dawley
Figure 1: Loss of FMRP protein in Fmr1 knockout rats FMRP protein expression is disrupted in Fmr1 knockout rats as compared to wild type controls as demonstrated by Western blot. Actin staining demonstrates equal sample loading.