This model contains a biallelic disruption of the Faah gene encoding the fatty acid amide hydrolase. FAAH is a serine hydrolase responsible for the breakdown of endocannabinoids. This model is useful for the study of cannabinoid biology including nociception.
- Diet Value
LabDiet #5R24 (RMH2500)
- Age Weight Curve
- Research Application
- Research Area
• Homozygous knockout rats exhibit complete loss of protein
• FAAH KO rats show an increased fear/startle reaction as observed in a functional observational battery (Irwin)
• FAAH KO rats show normal sensitivity to thermal pain via hot plate assay
• Background Strain: Sprague Dawley
- Figure 1
Figure 1: Western blot demonstrating lack of FAAH in FAAH knockout rats.
Brain homogenates were probed with anti-rat FAAH ab54615 (Abcam) at 1:500.
- Figure 2
Figure 2. Normal thermal sensitivity in FAAH KO rats.
FAAH KO rats display normal sensitivity to thermal pain as measured by hot plate assay.