Accurately track protein expression and localization in real-time at near physiological level
Horizon and Promega Corporation have partnered in a long-term relationship to develop a suite of best-in-class endogenous pathway tag and reporter cell lines for application in functional genomics research and drug discovery and development.
Gene-editing at the endogenous level allows researchers to examine near-physiological levels of protein and promoter activity. Traditional methods of understanding protein/promoter function have used exogenous plasmid-based over-expression systems which can result in artefactual results.
Our collection of ready-made cell lines with tags is growing. Alternatively, we can modify your specific cell line for you. To discuss design of your experiment or for more information on our reporter cell lines
Endogenous pathway tag and reporter cell lines allow researchers a much higher level of confidence that their experimental results accurately reflect the changes seen in real disease biology.
Horizon also offers a range of Turbo-GFP tagged isogenic cell line pairs for applications where rapid appearance of bright fluorescence is crucial, such as cell and organelle labelling or tracking promoter activity
This example shows HAP1 cells gene-edited to incorporate Turbo GFP at the endogenous level for LMNA, which is a nuclear membrane protein, and for TERF1 which locates to the telomeres in the nucleus, showing the expected patterns of localisation.
Here are some of the reporter cell line applications that the team at Horizon Discovery are experienced at working with:
Award winning tagged isogenic cell line pairs for applications where rapid appearance of bright fluorescence is crucial, such as cell and organelle labelling or tracking promoter activity
Our ready-made tagged organelle cell lines enable real time tracking of protein activity in live cells. These are a great alternative to unreliable antibodies.
The brightness of NanoLuc combined with its small size, high thermal stability, activity over a broad pH range, lack of post-translational modifications and uniform cellular distribution make it an ideal tool for detection of endogenous gene transcription and protein expression.
The HaloTag ligands are designed to covalently bind the HaloTag protein and come in a variety of types including fluorescent ligands and solid substrates, enabling researchers to use HaloTag in a wide variety of applications such as live cell imaging and isolation of HaloTag and therefore the protein of interest.