Bring the precision of CRISPR gene-editing technology to your lab without the hassle
Advantages of Horizon's gene-edited knockout cell lines:
Use the search bar at the top of the page to browse for your gene of interest, using the HUGO Gene Nomenclature. There are thousands of popular lines in stock - Your order could be delivered in only a few weeks!
Alternatively, if you can't find your cell line of interest, see our custom cell lines service:
See our guide to antibody validation
Guide RNAs, shown in orange, were designed upstream and downstream of the MALAT1 genomic locus. Clonal cell lines bearing the deletion can be identified by performing a PCR using primers (shown in green) flanking the deleted region. Primers (shown in blue) within the transcribed region, can be used to verify MALAT1 expression levels.
Validation of MALAT1 deletion clones. A PCR using primers flanking the deleted region corroborated the absence of the MALAT1 locus in both deletion clones, but not in the parental cells. The absence of MALAT1, both at a genomic and RNA level, was confirmed using primers within the transcribed region.
Using CRISPR Cas technology we can disable the gene of interest through the introduction of a frameshift mutation or by the removal of large fragments of the gene.
Frameshift mutations result in a small insertion or deletion (or InDel), via non-homologous end joining or NHEJ repair pathway in a coding exon. Consequently, this disrupts the gene at the endogenous level. This is a well-established method that results in clonal cell lines where the targeted gene is inactivated.
The schematic diagram illustrates how Cas9 cleavage can introduce a frameshift mutation (in this case a small deletion) resulting in a non-functional gene.
The absence of protein can be confirmed by western blot, as shown for these frameshift knockout cell lines (α).
Deletions are an alternative method to disable gene function, particularly useful for where the small insertion or deletion that is produced by a frameshift is usually not sufficient to block function.
Horizon now offers the generation of cell lines in which larger defined regions are excised by CRISPR-Cas9, combining two guide RNAs and screening for clones that lack the intervening DNA sequence to ensure that the gene is disrupted.
This is particularly useful for non-coding parts of the genome:
For more information, or to get advice from our team: