Our HAP1 cell line database represents the synergy of two great technologies - the amenability of human haploid HAP1s to gene editing and CRISPR CAS9.
Definition: Haploid cells have a single copy of almost every human chromosome. HAP1 is a human near-haploid cell line derived from the male chronic myelogenous leukemia (CML) cell line KBM-7.
The HAP1 cell line properties include:
The majority of commonly used cell lines are diploid or even have multiple copies of any particular allele. When gene editing for loss of function mutations, each allele has to be modified for the phenotype to be expressed.
The HAP1 cell line has the advantage of having one copy of each gene meaning you can be sure the editied allele will not be masked by addition alleles. This is is why haploid cells have been an established tool for genetic analysis for decades, as only one allele requires modification.
As the HAP1 is human in origin this represent a significant advancement in the field.
Spectral karyotyping and brightfield imaging of HAP1 cells
The HAP1 cell line is a versatile cell line with many applications.
Explore our range of ready-made and custom cell lines:
Find out more about the types of genomic modifications and their editing efficiencies in this recorded webinar:
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