Cell Line Applications

Horizon Isogenic human cell line pairs provide a model system for the study of the role of genes in cellular processes and drug responses. Cell lines are provided with a matched wild type control parental cell line ensuring phenotypes can be attributed directly to the requested genetic modification(s). Some common uses of cell lines are:

  • Study loss or gain of function mutations at the endogenous level
  • Initiate target identification and validation studies
  • Identify responsive or resistant patient populations in a human cell line model
  • Analyse expression, protein interactions and localization using endogenously tagged genes

These models complement and surpass non isogenic cell lines where findings are difficult to understand due to the absence of a normal control cell.

Using Horizon Cell Lines for Functional Studies
Cell line applications

Olaparib sensitivity in normal and BRCA2 (-/-) Knockout Cell Lines; Cell Lines allow the role of specific genes to be studied without the influence of other factors.

The data shown demonstrates the sensitivity of BRCA2 knockout cell lines to Olaparib, a PARP inhibitor compared to Gemcitabine a chemotherapy drug.

Differential sensitivities to EGFR inhibitors in normal and EGFR Knockin Cell Lines; Isogenic cell lines allow the role of specific genes to be studied without the influence of other factors. The data above shows the anti-proliferative effects of the EGFR inhibitors gefitinib and erlotinib on cell lines containing different engineered EGFR mutants [EGFR ∆E746-A750; EGFR L858R, EGFR T790M] in 96hr proliferation assays.

Finding Your Ideal Cell Line

Our cell line range is constantly growing. For our most up-to-date list of ready-made cell lines and details of custom made engineering projects, you can browse our cell line range via the following link.

Cell Line Database

We are so confident you'll be happy with our catalog cell lines, for a limited time, register your interest and we'll even provide a Money Back Guarantee.

For offline access, many of our popular cell lines that are usually available for immediate delivery are listed in our downloadable catalog - your gene edited cell line may have already been created!

Cell Line Catalog Download

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