BRAF V600E cfDNA Reference Standard Set

Format
cfDNA

Description

The BRAF V600E cfDNA Reference Standards are highly-characterized, biologically-relevant quality control materials used to assess the performance of cfDNA assays that detect BRAF V600E somatic mutations, including quantitative PCR, next-generation sequencing, and other novel assays.

With this panel you are able to:
• Analyze the sensitivity of your assay
• Gain certainty of the limit of detection
• Optimize and validate new protocols and routinely monitor the performance of your assay

Catalog ID
Qty
Unit Price
HD781
$480.00
Bulk order discount

Availability: On Backorder

Dispatched within 24 hours

Total:
OR
Technical Information

Horizon cfDNA standards eliminate the variability associated with patient-derived reference standards, and avoid the hassle of sourcing, characterizing, and documenting your own biofluids or cell line mixes. The BRAF V600E cfDNA reference standards are provided as a Set at allelic frequencies of 5%, 1%, and 0.1%.

Horizon’s cfDNA products are all derived from human cell lines, and are fragmented to mimic the 160 bp average fragment size of patient-derived cfDNA. This format ensures that they may applied to a wide-range of cfDNA assays including quantitative PCR and next-generation sequencing. Batch-specific copy number values measured via ddPCR are provided with each Set.


Technical Data

Genes Covered
BRAF
Cosmics
1,500+
Allelic Frequencies
5%, 1%, 0.1% and 100% wild type
DNA Base Change
GTG→GAG
NCBI Reference Assembly SNP
rs113488022
Cosmic ID
COSM476
Buffer
Tris-EDTA (10mM Tris-HCl, 1mM EDTA), pH 8.0

Product Information

Fragment Size
160 bp
Unit Size
350 ng per vial
Concentration
40 ng/µl
Presence confirmed in parental cell line
For information on endogenous mutations in these standards, you may download whole-exome sequencing data of the parental cell lines here: Click here to view exome sequencing data
Verified Mutations
100% BRAF Wild Type cfDNA Reference Standard (Part No.: HD772)
ChromosomeGeneVariantExpected Allelic Frequency
7q34BRAFV600E0%
5% BRAF V600E cfDNA Reference Standard  (Part No.: HD773)
ChromosomeGeneVariantExpected Allelic Frequency
7q34BRAFV600E5%
1% BRAF V600E cfDNA Reference Standard   (Part No.: HD774)
ChromosomeGeneVariantExpected Allelic Frequency
7q34BRAFV600E1%
0.1%  BRAF V600E cfDNA Reference Standard (Part No.: HD775)
ChromosomeGeneVariantExpected Allelic Frequency
7q34BRAFV600E0.1%

General Information

Storage
4°C
Expiry
12 months from the date of manufacture
Cell Line Background

HCT116

Quality Control

Fragmentation Size
D1000 DNA ScreenTape assay
Allelic Frequency
Droplet Digital™ PCR
Genotype
BRAF (V600E/+)
Quantification
Qubit dsDNA BR Assay (post-fragmentation)
Protocols & Documents

Please browse our collection of downloads below.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Certificate of Analysis

Batch ID: 16923
PDF Download
Batch ID: 17674
PDF Download

Documents & Related Literature

Gene Editing Sequence File
PDF Download
Product Specification
PDF Download
Safety Data Sheet
PDF Download
Order & Delivery Information

Please see our terms and conditions of ordering.

If you need any support, advice or additional documentation then don't hesitate to contact us here.

Order Information

We accept orders using the following methods:

Online: 

Our online catalog and ordering system can be used to search our full menu of available HDx™ Reference Standards. Select your desired reference standards and add them to the shopping basket. Your order can be submitted instantly using credit card, telephone ordering or a purchase order number as the preferred payment method.

Offline:

If you prefer to send us orders outside our online ordering system, please see the Contact Us page for telephone, fax, and email details in order to place an order.  We recommend that you use our online system to identify the products you require and their respective “HD” product codes. To order offline we will require the following information:

  • Product code(s) of the item(s) you wish to order (e.g. "HD123")
  • Your name, email address, title and organization
  • Shipping address and billing address with contact names
  • Purchase Order number if you have one
  • Telephone number
  • VAT Number (European Countries only)

Shipping Information

We aim to ship your order direct within 5 business days of receipt of your order and we will notify you of your expected delivery date. We ship direct to the following territories:

TerritoryPrice (Room Temperature)Price (4°C)Service

US & Canada

$25.00

$45.00

FEDEX International Priority

EU Countries
(Excluding Italy)

€20.00

€36.00

FEDEX International Priority

UK

£18.00

£32.40

Royal Mail Recorded Signed For™

Rest of the World
(Excluding Japan, Israel, Hong Kong) 

 

$25.00 - $80.00

 

$45.00 - $120.00

 

FEDEX International Priority

 

We have selected Room Temperature as the most appropriate shipping condition to ensure the stability of our HDx DNA and FFPE Reference Standards and 4°C for our HDx FISH Reference Standards during transit.

It is possible to use your own FEDEX account for shipment of goods and we will apply a small fee ($5.00 / £5.00 / €5.00) to cover packaging costs for Room Temperature Shipments and ($25.00 / €20.00 / £18.00) for Dry Ice Shipments. 

If you’re having trouble ordering or finding what you’re looking for, or if you require something we don’t yet offer, please see below for more information: 

Contact Us Terms of Ordering

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