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Sirion Services

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Sirion Custom Services


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The RNAiONE platform identifies the 10 most promising shRNA sequences against your gene of interest using proprietary in silico algorithms and validates the knockdown efficiency for each via qRT-PCR in an optimized cell-based system. You will receive a report containing the nucleotide sequence and performance of the 10 shRNAs.

Sirion takes the best performing shRNA (discovered using the RNAiONE platform) and clones into a lentiviral vector. Cloning success is verified by sequencing and you will receive 1 x 108 viral particles, ready to transduce into your cell line of choice.

Cell Pool generation consists of both the RNAiONE stage and the lentiviral generation, further transducing cell lines to generate stable knockdown cell pools. Validation is through qRT-PCR and you will receive >1 x 106 cells containing the high-efficiency gene knockdown.


What is your gene of interest?

Gene ID

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Gene name

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Which mode of shRNA expression?

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Constitutive expression

TET-inducible expression (TET-on)

TET-inducible expression (TET-off)


Are you providing cDNA?

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Yes

No

Name of third-party vendor (if known). Note that there is a $250 fee for this service.


Cost

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Thank you for registering to the Horizon Discovery website. We hope you enjoy and benefit from our online resources, and please feel free to contact us if you have any queries or feedback through the website itself or at info@horizondiscovery.com.


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