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GENASSIST™ Custom Gene Editing Kits

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CRISPR and rAAV enabled tools and services

To support customers’ efforts to generate new cell lines in their labs, Horizon has launched a broad and flexible range of robust and validated tools and services designed to enable any user to efficiently address any gene editing project. These include off the shelf plasmids; custom guide RNA design and validation, and platform cell lines designed to accelerate further modifications. We also offer high-level scientific support for customers who choose to benefit from expert guidance.


Organization type

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CRISPR/Cas9 plasmids

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Catalog ID Description Plasmid Cost Quantity Viral Packaging Add to order
CA2-001A Plasmid donor for insertion of Cas9 nickase D10A into Human Rosa26 locus, transformation volume $ 200.00 1000ng
CA2-001B Plasmid donor for insertion of Cas9 nickase D10A into Human Rosa26 locus, transfection volume $ 375.00 5000ng
GE10-001A Expression plasmid for a single guide RNA targeting Human Rosa26 locus, transformation volume $ 200.00 1000ng
GE10-001B Expression plasmid for a single guide RNA targeting Human Rosa26 locus, transfection volume $ 375.00 5000ng
GFP-001A GFP Transfection control plasmid $ 80.00 1000ng
PL1-001A Expression plasmid for wild type Cas9 (CMV promoter), transformation volume $ 200.00 1000ng
PL1-001B Expression plasmid for wild type Cas9 (CMV promoter), transfection volume $ 375.00 5000ng
PL4-001A Expression plasmid for Cas9 nickase D10A (CMV promoter), transformation volume $ 200.00 1000ng
PL4-001B Expression plasmid for Cas9 nickase D10A (CMV promoter), transfection volume $ 375.00 5000ng
CA5-001B Plasmid donor for insertion of Cas9 nickase D10A into Mouse Rosa26 locus, transfection volume $ 375.00 5000ng
GE14-001A Expression plasmid for a single guide RNA targeting Mouse Rosa26 locus, transformation volume $ 200.00 1000ng
GE14-001B Expression plasmid for a single guide RNA targeting Mouse Rosa26 locus, transfection volume $ 375.00 5000ng
CA5-001A Plasmid donor for insertion of Cas9 nickase D10A into Mouse Rosa26 locus, transformation volume $ 200.00 1000ng

Guide Solution

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Catalog ID Description Plasmid Cost Add to order
GE12-001A Cloning plasmid for single guide RNA (U6 promoter) $ 200.00
GE6-001A Cloning plasmid for single guide RNA (U6 promoter), expressing wild type Cas9 (CMV promoter) $ 200.00
GE9-001A Cloning plasmid for dual guide RNA (U6 promoter), expressing Cas9 nickase D10A (CMV promoter) $ 300.00
GFP-001A GFP Transfection control plasmid $ 80.00
GE13-001A Cloning plasmid for dual guide RNA (U6 promoter) $ 200.00

What is your gene of interest?

Gene ID

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Gene name

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Knock out or Knock in?


Sequence

Between 20 and 250 base pairs

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Additional information

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Donor type

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Sequence

Between 20 and 250 base pairs

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Single or Dual? * required

Generation of a single guide suitable for use with wild-type Cas9 for high efficiency cutting or two guides suitable for use with Cas9 nickase to generate staggered nicks in DNA for better specificity and efficient cutting.

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Choose format * required

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How many clones?

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If you would like to receive additional guides for your target in expression plasmids, enter the TOTAL number of plasmids that you would like to receive.


As a standard, Horizon clones guides into our 'All in one' plasmids that combine Cas9 with guide expression. If you would prefer your guide in a different backbone Horizon has available, please describe your preference below.


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Would you like your plasmid to come packaged in Adenovirus (ideal for hard to transfect cells)?

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Species * required

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Do you require validation?

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Donor

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Catalog ID Description Plasmid Cost Add to order
DO1-001A Plasmid donor for Scarless insertion $ 200.00
DO2-001A Plasmid donor for insertion of a DNA sequence and a drug selection cassette (Neomycin) $ 200.00
DO3-001A Plasmid donor for insertion of a DNA sequence and a GFP expression cassette $ 200.00
GFP-001A GFP Transfection control plasmid $ 80.00
DO2-002A Plasmid donor for insertion of a DNA sequence and a drug selection cassette (Puromycin) $ 200.00

What is your gene of interest?

Gene ID

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Gene name

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Donor complexity * required

<200 base pairs

>200 base pairs

More complex donors including those with long homology arms, reporter donors, etc. are >200 base pairs. For simple oligo donors, choose <200 base pairs.


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How will you supply your sequence to Horizon? * required


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Please enter your sequence (<200 base pairs)

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Choose format * required

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Would you like your plasmid to come packaged in Adenovirus (ideal for hard to transfect cells)

$ 0.00

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Do you want additional support from Horizon?

Yes

No


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Species * required

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Reporter Donor?

Yes

No


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Select one of the following

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Please choose the reporter configuration you are interested in. For more information, see the X-MAN™ Reporter Kits section of this website



Selection & Markers


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Additional information

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Cell line

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QuickStart Cell Lines

Name Catalog ID Description Rights to generate
Add to order
CRISPR Ready - Rosa26 CMV Cas9n HD 175-006 HEK293 Rosa26-CMV Cas9 nickase - Cell line derived from HEK293 expressing Cas9 nickase D10A inserted into the Rosa26 locus
Learn more  

 

X-MAN™ Gene of interest

Enter all genes of interest separated by spaces


X-MAN™ Cell Lines

Name Catalog ID Description License Type License Duration Add to order

Tick box to confirm you would like the Right to Modify X-MAN cell line(s) * required



Services & Support

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How many pre-paid hours do you require?
Information on discounts that apply at certain amounts
Cost includes any support requested in the sections above.

Cost

Hours


 

Free Hours



How do you anticipate using this support?

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Would you like to license your cell line back to Horizon?

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Horizon Discovery is interested in licensing many of the cell lines that are being developed by our customers in return for a royalty. There is no commitment from you to do so.


Cost

£00.00

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