Horizon and Promega Corporation have partnered in a long-term relationship to develop a suite of best-in-class endogenous pathway tag and reporter cell lines for application in functional genomics research and drug discovery and development.
X-MAN™ Reporter Kits enable functional cell-based assays to be carried out at the endogenous level, looking at natural levels of protein and promoter activity. This affords researchers a higher level of confidence that their experimental results are meaningful, more accurately reflecting the changes seen in real disease biology.
Two classes of X-MAN™ Reporter Kits are available:
NanoLuc™ luciferase is a novel, small, bright luciferase reporter. It is one third the size and 100 times brighter than the most commonly used luciferase, firefly. Reporter cell lines involve knock-in of the Promega NanoLuc™ reporter into an endogenous locus for in vitro and in vivo pathway read-outs and protein stability experiments. This brightness allows the interrogation of pathway activity from endogenous genes/ promoters, expanding the range of questions that can be asked and the range of genes that can be studied.
Kits are available in 10, 100, 500, 1,000 ml and custom reporter volume sizes.
X-MAN™ NanoLuc® Options and Pricing
X-MAN™ NanoLuc™ Reporter Kit pricing is based on the volume of reagent required for your experimental needs:
Reagent Volume
Assays Possible with Kit
Price
10ml
100 (in 96-well plates)*
$1,600
100ml
1,000 (in 96-well plates)*
$8,200
500ml
5,000 (in 96-well plates)*
$16,000
1,000 ml
10,000 (in 96-well plates)*
$24,000
Custom
Contact us
* x4 assays in 384 well plates; x16 in 1,536 well plates
Terms of reporter kit licenses are for one year or until the reagents included in the kit are consumed, whichever comes first.
X-MAN™ NanoLuc™ kits are supplied with:
A vial of the cell line of interest, and
All reagents and buffer necessary to perform a set of experiments
Some kits have an optional Protein Reporter Control cell line available which can be included in the Kit for an additional $800.
X-MAN™ NanoLuc® Reporter Kit Product Range
X-MAN™ NanoLuc™-PEST Promoter Kit
Has a short intracellular lifetime and
Shows reduced accumulation in cells due to the presence of the PEST sequence which targets NlucP protein for degradation
NanoLuc™ luciferase detection at endogenous expression levels using HCT116 HIF1A NanoLuc™ - PEST promoter fusion cell lines, (A) correlation of cell number with luciferase signal; (B) correlation of concentration of Actinomycin D, a non-specific transcriptional inhibitor, with luciferase signal.
Flexible and efficient system….Wider experimental range…Reliable results
HaloTag® is an extremely flexible reporter, enabling protein imaging and localization (which can be performed in live cells), protein-protein interaction experiments through protein pull-down, or protein purification, all using the same cell line by choosing different ligands with which to interrogate the cell line. This approach avoids the use of antibodies, which are often not available or of poor quality.
Kits are available in 15 and 30 µl reagent volume sizes for imaging ligands - two are available, TMR (for red fluorescing) and Oregon Green), or in 24 reactions for pull-down ligands.
X-MAN™ HaloTag® Options and Pricing
X-MAN™ HaloTag® Reporter Kit pricing is based on the volume of reagent required for your experimental needs:
Ligand
Reagent Volume
Assays Possible with Kit
Price
Imaging (TMR or Oregon Green)
15 µl
150 (in 96-well plates)*
$4,000
Imaging (TMR or Oregon Green)
30 µl
300 (in 96-well plates)*
$7,000
Protein Pull-Down
24 Reactions
$6,000
* x4 assays in 384 well plates; x16 in 1,536 well plates
Terms of reporter kit licenses are for one year or until the reagents included in the kit are consumed, whichever comes first.
X-MAN™ HaloTag® kits are supplied with:
A vial of the cell line of interest
Its parental cell line which can act as a control, and
All reagents and buffer necessary to perform a set of experiments
Some kits have an optional Protein Reporter Control cell line available which can be included in the Kit for an additional $800.
X-MAN™ HaloTag® Reporter Kit Product Range
Engineered to generate a C-terminal fusion of HaloTag® to the endogenous protein of interest
Ideal for studying a wide variety of properties of the protein of interest due to the broad range of HaloTag® ligands available
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