LOCUS       pENTR201gb              2274 bp    DNA     circular UNA 14-JUL-2020
DEFINITION  pENTR201 Gateway entry vector.
ACCESSION   urn.local...11-c5uts7j
VERSION     urn.local...11-c5uts7j
KEYWORDS    .
SOURCE      Invitrogen Life Technologies
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     terminator      complement(80..107)
                     /label="rrnB T2"
     terminator      complement(238..282)
                     /label="rrnB T1"
     primer_bind     307..331
                     /Sequence="TCGCGTTAACGCTAGCATGGATCTC"
                     /Hairpin_Tm="61.4"
                     /Tm="67.4"
                     /Self_Dimer_Tm="41.7"
                     /%GC="52.0"
                     /created_by="primer3"
                     /label="pDONR201-forward"
     misc_feature    338..437
                     /label="attL1"
     misc_feature    443..452
                     /label="linker-ORG-linker"
     misc_feature    complement(457..556)
                     /label="attL2"
     primer_bind     complement(580..603)
                     /Sequence="GTAACATCAGAGATTTTGAGACAC"
                     /Hairpin_Tm="None"
                     /Tm="58.1"
                     /Self_Dimer_Tm="None"
                     /%GC="37.5"
                     /created_by="primer3"
                     /label="pDONR201-reverse"
     CDS             679..1486
                     /label="KanR"
     rep_origin      1615..2234
                     /label="pUC origin"
ORIGIN      
        1 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gctagccagg
       61 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg
      121 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt
      181 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat
      241 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc
      301 ctactctcgc gttaacgcta gcatggatct cgggccccaa ataatgattt tattttgact
      361 gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt
      421 gtacaaaaaa gctggcacca tgnnnnnnnn nnggcgaccc agctttcttg tacaaagttg
      481 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
      541 ataaaatcat tatttgccat ccagctgcag ctctggcccg tgtctcaaaa tctctgatgt
      601 tacattgcac aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac
      661 agtaatacaa ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat
      721 tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca
      781 ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat
      841 ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg
      901 gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta
      961 ctcaccactg cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca
     1021 ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt
     1081 tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg
     1141 aataacggtt tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa
     1201 caagtctgga aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat
     1261 ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat
     1321 gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc
     1381 ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct
     1441 gatatgaata aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt
     1501 aattggttgt aacactggca gagcattacg ctgacttgac gggacggcgc aagctcatga
     1561 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1621 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1681 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1741 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     1801 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1861 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1921 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1981 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2041 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2101 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2161 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     2221 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctc
//