LOCUS       dna                      2990 bp                                   
FEATURES             Location/Qualifiers
     Rep_Origin      138..444
                     /gene="f1 origin"
     Reporter        462..622
                     /gene="lacZ-alhpa reporter"
     Promoter        626..645
                     /gene="T7 prom"
     misc_binding    669..676
                     /dbxref="REBASE:NotI"
     Other Gene      677..710
                     /gene="loxP other"
     misc_binding    736..741
                     /dbxref="REBASE:EcoRI"
     misc_binding    748..753
                     /dbxref="REBASE:BamHI"
     misc_binding    782..787
                     /dbxref="REBASE:ApaI"
     misc_binding    788..793
                     /dbxref="REBASE:KpnI"
     Promoter        804..823
                     /gene="T3 prom"
     Promoter        887..970
                     /gene="lac prom"
     Rep_Origin      1231..1850
                     /gene="pUC origin"
     misc_binding    1596..1604
                     /dbxref="REBASE:AlwNI"
     Marker          2005..2865
                     /gene="AmpR marker"
     Promoter        2907..2945
                     /gene="Bacterial-Promoter prom"
BASE COUNT    760 a   738 c   761 g   731 t    0 others
ORIGIN
        1 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag     
       61 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac     
      121 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga     
      181 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc     
      241 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg     
      301 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa     
      361 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac     
      421 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct     
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa     
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg     
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca     
      661 ccgcggtggc ggccgcataa cttcgtatag catacattat acgaagttat ggatcaggcc     
      721 aaatcggccg agctcgaatt cgtcgaggga tccggccata agggcctgat ccgtcgaggg     
      781 ggggcccggt accagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc     
      841 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg     
      901 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat     
      961 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg     
     1021 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct     
     1081 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc     
     1141 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg     
     1201 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg     
     1261 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg     
     1321 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac     
     1381 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca     
     1441 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt     
     1501 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc     
     1561 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag     
     1621 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac     
     1681 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt     
     1741 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     
     1801 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg     
     1861 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa     
     1921 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat     
     1981 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc     
     2041 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat     
     2101 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc     
     2161 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc     
     2221 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag     
     2281 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg     
     2341 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg     
     2401 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag     
     2461 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt     
     2521 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga     
     2581 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc     
     2641 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc     
     2701 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc     
     2761 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc     
     2821 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca     
     2881 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat     
     2941 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc                
//